Fig. 5

LPS-induced NF-κB signaling and expression of inflammatory mediators are associated with primary cilia. HT22 cells were transfected with control (scrambled) siRNA or siRNA targeting Kif3a for 24 h before exposure to LPS (100 ng/ml). Six hours after LPS treatment, cells were harvested and stained for detection of ARL13B (a, b), phosphorylated p65 (P-p65) (a, c), IκBα (a, d), Cox2 (a, e), and iNOS (a, f). The data are normalized to β-actin and quantified and expressed as optical densities and are presented as the mean ± SEM of three independent experiments. *p < 0.05, ***p < 0.001