Fig. 1

Caspase-1 activation and necrotic cell death were induced in the early stages after SAH. a Representative western blot and the relative band densities of caspase-1 p20 in the ipsilateral cortex at different time points (6, 12, 24, 48, and 72 h) after SAH (n = 4/group). The bar represents the mean ± SD. *P < 0.05 vs sham, **P < 0.01 vs sham. b Representative photomicrographs and the quantification of the PI-positive neural cells in the ipsilateral cortex at 24 h after SAH (n = 3/group). Fluorescence colors: DAPI: blue and PI: red. Scale bar = 100 μm, the quantification of the PI-positive neural cells expressed as positive cells per square millimeter. The bar represents the mean ± SD. **P < 0.01 vs sham