Fig. 7

Autophagy inhibitor 3-MA abolishes the neuroprotective effects of fluoxetine on necrotic cell death, brain edema, and neurological function after SAH. a Representative photomicrographs of the PI-positive neural cells in different groups at 24 h after SAH (n = 3/group). Fluorescence colors: DAPI: blue and PI: red. Scale bar = 100 μm. b The quantification of the PI-positive neural cells expressed as positive cells per square millimeter. **P < 0.01 vs the sham group. ^## P < 0.01 vs SAH + vehicle group. ^& P < 0.05 vs SAH + fluo group. c Quantification of brain water content (n = 6). The bars represent the mean ± SD. **P < 0.01 vs the sham group. ^## P < 0.01 vs SAH + vehicle group. ^& P < 0.05 vs SAH + fluo group. d Quantification of neurological scores (n = 15/group). The bars represent the mean with interquartile range. *P < 0.05 vs the sham group. ^# P < 0.05 vs SAH + vehicle group. ^& P < 0.05 vs SAH + fluo group