Fig. 6From: Activation of dorsal horn cannabinoid CB2 receptor suppresses the expression of P2Y12 and P2Y13 receptors in neuropathic pain ratsThe effect of AM1241, SB203580, and PDTC on the thermal threshold and the expression of P2Y12 and P2Y13 receptors. Data are presented as TWL. All values represent mean ± standard deviation. a Comparison of thermal stimulation pain threshold in the ipsilateral hind paw of rats (n = 8). Spinal ADPbetaS injection reduced TWL from days 3 to 7 after ADPbetaS injection. *P < 0.05 compared with control group. Pretreated with AM1241 (100 PM, 20 min), SB203580 (50 μM), or PDTC (5 μg/10 μl) significantly inhibited the reduced withdrawal threshold days 3 to 7 in ADPbetaS-treated rats, respectively (compared with ADPbetaS-treated rats, AM1241: P < 0.05; SB203580: P < 0.05; PDTC: P < 0.05). b RT-PCR results show the expression of P2Y12 mRNA expression in the dorsal spinal cord of the rats in every groups (n = 8 per group). *P < 0.05 means comparison with the control group; + P < 0.05 means comparison with the ADPbetaS-treated rats. c RT-PCR results show the expression of P2Y13 mRNA expression in the dorsal spinal cord of the rats in every group (n = 8 per group). *P < 0.05 means comparison with the control group; + P < 0.05 means comparison with the ADPbetaS-treated rats. d Western blotting image of P2Y12R expression. The top panel was the target band, P2Y12R, and the bottom one was for the loading control beta-actin. e Western blotting quantitative analysis of the P2Y12R expression in the dorsal spinal cord of the rats. *P < 0.05 means comparison with the control; + P < 0.05 means comparison with the ADPbetaS-treated rats. f Western blotting quantitative analysis of the P2Y13R expression in the dorsal spinal cord of the rats. *P < 0.05 means comparison with the control; + P < 0.05 means comparison with the ADPbetaS-treated ratsBack to article page