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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Activation of dorsal horn cannabinoid CB2 receptor suppresses the expression of P2Y12 and P2Y13 receptors in neuropathic pain rats

Fig. 6

The effect of AM1241, SB203580, and PDTC on the thermal threshold and the expression of P2Y12 and P2Y13 receptors. Data are presented as TWL. All values represent mean ± standard deviation. a Comparison of thermal stimulation pain threshold in the ipsilateral hind paw of rats (n = 8). Spinal ADPbetaS injection reduced TWL from days 3 to 7 after ADPbetaS injection. *P < 0.05 compared with control group. Pretreated with AM1241 (100 PM, 20 min), SB203580 (50 μM), or PDTC (5 μg/10 μl) significantly inhibited the reduced withdrawal threshold days 3 to 7 in ADPbetaS-treated rats, respectively (compared with ADPbetaS-treated rats, AM1241: P < 0.05; SB203580: P < 0.05; PDTC: P < 0.05). b RT-PCR results show the expression of P2Y12 mRNA expression in the dorsal spinal cord of the rats in every groups (n = 8 per group). *P < 0.05 means comparison with the control group; + P < 0.05 means comparison with the ADPbetaS-treated rats. c RT-PCR results show the expression of P2Y13 mRNA expression in the dorsal spinal cord of the rats in every group (n = 8 per group). *P < 0.05 means comparison with the control group; + P < 0.05 means comparison with the ADPbetaS-treated rats. d Western blotting image of P2Y12R expression. The top panel was the target band, P2Y12R, and the bottom one was for the loading control beta-actin. e Western blotting quantitative analysis of the P2Y12R expression in the dorsal spinal cord of the rats. *P < 0.05 means comparison with the control; + P < 0.05 means comparison with the ADPbetaS-treated rats. f Western blotting quantitative analysis of the P2Y13R expression in the dorsal spinal cord of the rats. *P < 0.05 means comparison with the control; + P < 0.05 means comparison with the ADPbetaS-treated rats

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