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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Increased frequency of IL-6-producing non-classical monocytes in neuromyelitis optica spectrum disorder

Fig. 2

Intracellular staining of healthy control (HC), MS, and NMOSD patient monocytes. Monocytes purified with MACS pan-monocyte isolation kit were treated with or without CD40L (1.0 μg/mL) for 6 h and stained with antibodies to the appropriate surface antigen and anti-cytokine antibodies (TNFα, IL-1β, IL-6, IL-23, IL-10). The graphs shown are representative graphs (a) and show a comparison between three groups (n = 15) (b). Graphs were created using Flowjo and Prism software. Assessment of statistical significance was performed by two-way ANOVA. Data are mean ± S.E.M of 15 subjects each. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. US unstimulated, I interferon-γ, 40 L CD40L

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