Fig. 6

The effects of AG490 on TNF-α and IL-6 production induced by Hcy. a, b TNF-α (green) and IL-6 (red) immunoreactivity in the DG region of the rat hippocampus and the cerebral ischemic cortex after Hcy and AG490 treatment. The blue staining represents DAPI. Scale bar = 50 μm. c-f Graph showing the mean fluorescence intensity for TNF-α and IL-6 (n = 6, 10 fields/brain). g Representative bands of TNF-α and IL-6 by western blot analysis. β-actin protein was used here as an internal control. h Bar graphs show semiquantitative levels of TNF-α and IL-6 as determined by band density analysis. The data are presented as the mean ± SD from four independent experiments. ^a p < 0.05 vs. the SHAM group, ^b p < 0.05 vs. the MCAO group