Fig. 6

Redox status alters microglia morphological phenotypic changes. Differential interference contrast (DIC) images of EOC13.31 (20×), cells were either untreated (control) or pretreated with 500 μM NAC or 200 μM BSO for 18 h, prior to 22 mM EtOH exposure for 6 h. (a). Elongation factor was calculated by measuring the length of the long axis divided by the length of the short axis (b). Values obtained were divided into three categories, and a reference image of each category is depicted along the Y-axis. qRT-PCR was conducted of selected M1 (IL-1β, IL-6, CCL9) and M2 (IL-10, CCL2) markers. Expression level of each gene was normalized to 18S rRNA and illustrated relative to the control group (c). Values are presented as mean ± SEM of three independent experiments (*p ≤ 0.05; **p ≤ 0.005)