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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Acute maternal oxidant exposure causes susceptibility of the fetal brain to inflammation and oxidative stress

Fig. 7

Oxidative stress and intracellular GSH dictate M1/M2 phenotypic acquisition of microglia. EOC 13.31 cells grown on coverslips were pretreated with either NAC (500 μM) or BSO (200 μM) for 18 h, followed by either absence or presence of EtOH (22 mM) exposure for 6 h. Immunohistochemistry was performed using antibodies against IL-1β (M1), Arginase1 (M2) and subsequently visualized with Alexa Fluor 488 (green) and Alexa Fluor 647 (red) labeled secondary antibodies, respectively. DAPI was used as a nuclear stain. Images were captured with a confocal microscope using a 60× objective. Scale bar = 40 μm. Images shown are representative from three independent experiments

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