Fig. 8

Suppression of GSH triggers NF-κB activation both in in vitro and in vivo. EOC cells were either untreated (control) or pretreated with 500 μM NAC or 200 μM BSO for 18 h, prior to 22 mM EtOH exposure for 6 h. Western blot analysis was performed to determine the expression and activation of NF-κB p105 (a) phosphorylated p65 at ser⁵³⁶ and total p65 protein (b). β-actin served as a loading control. In similar experiments, nucleus and cytosol fractions were separated to determine expression levels of p50 (c). Lamin B and β-actin served as nuclear and cytosolic loading control, respectively. Representative immunoblot of p105 in fetal brain samples taken 24 h after dams were pretreated 1 h with either NAC (4 mg/dose) or BSO (1.5 g/kg) followed by ethanol exposure (2.5 g/kg, s.c) (d)