Fig. 3
β3 Integrin expression levels affect Thy-1-induced astrocyte migration. a β3 Integrin knock-down reduces Thy-1-induced astrocyte migration. β3 Integrin levels and wound-healing assay quantification in β3 Integrin knock-down, TNF-treated astrocytes stimulated with Trail-R2-Fc or Thy-1-Fc. b Over-expression of β3 Integrin is sufficient to allow Thy-1-induced astrocyte migration. β3 Integrin levels and wound-healing assay quantification in β3 Integrin over-expressing astrocytes stimulated with Trail-R2-Fc or Thy-1-Fc. c Western blot of proteins of the Thy-1-induced signaling pathway in astrocytes over-expressing GFP-tagged β3 Integrin. d Wound-healing assay in TNF-treated astrocytes stimulated with Thy-1(RLE)-Fc, a Thy-1-Fc mutant that lacks the β3 Integrin-binding domain. e Western blot of markers of reactivity in astrocytes over-expressing GFP-tagged β3 Integrin. β actin is used as a loading control. Values shown in graphs are the means ± s.e.m. from three independent experiments. *p < 0.05; ***p < 0.001