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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Agonists for G-protein-coupled receptor 84 (GPR84) alter cellular morphology and motility but do not induce pro-inflammatory responses in microglia

Fig. 6

Motility analysis of 6-OAU-treated microglia from DBA/2 mice. a DNA sequencing shows a frame-shift deletion in exon 2 of GPR84 from DBA/2 mice. b, c Microglia from DBA/2 mice were exposed to vehicle and 1 μM 6-OAU. After 2-min incubation, cells were fixed and stained with phalloidin (red) and DAPI (blue) to detect actin polymerization and nuclei, respectively. Scale bar, 20 μm. d, e Overlay of individual microglial trajectories after vehicle or 6-OAU treatment for 30 min (n = 5 each). f, g MetaMorph-based quantification of distance and velocity of cell migration. Cells were tracked over 30-min periods. Data represent mean ± SEM of 10 cells per group

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Journal of Neuroinflammation

ISSN: 1742-2094