Fig. 3

RGS2 expression after exposure to inflammatory cytokines. qPCR was performed on samples after 7 days in FBS 3%-medium in the presence of 2, 10, or 50 ng/mL of IL-1β (a, d), TNFα (b, e) or IL-6 (c, f). Cytokines were added during the 4, 8, 24, or 48 last hours of maturation. RGS2 mRNA expression was normalized to GAPDH. Each experiment was conducted independently. The FBS 3% condition was used as control. Data are presented as mean ± SEM; n = 6; ANOVA one-way with Dunnett’s post test; **p < 0.01, ***p < 0.001 (vs control). F _3,30 = 4.799 (IL-1β 4 h), 6.214 (IL-1β 8 h), 0.5637 (TNFα 4 h), 4.517 (TNFα 8 h), 1.609 (IL-6 4 h), and 0.2002 (IL-6 8 h). For 24 and 48 h, experiments were conducted independently and compared with a Student’s t test for which a p value < 0.05 was defined as significant