Fig. 5

RGS4 expression after exposure to inflammatory cytokines. qPCR was performed on samples after 7 days in FBS 3%-medium in the presence of 2, 10, or 50 ng/mL of IL-1β (a, d), TNFα (b, e), and IL-6 (c, f). Cytokines were added during the 4, 8, 24, and 48 last hours of maturation. RGS4 mRNA expression was normalized to GAPDH expression. Each experiment was conducted independently. The FBS 3% condition was used as control. Data are presented as mean ± SEM; n = 6; ANOVA one-way with Dunnett’s post test; *p < 0.05, **p < 0.01 (vs control). F _3,30 = 2.425 (IL-1β 4 h), 3.825 (IL-1β 8 h), 1.365 (TNFα 4 h), 4.134 (TNFα 8 h), 1.173 (IL-6 4 h), and 6.218 (IL-6 8 h). For 24 and 48 h, experiments were conducted independently and compared with a Student’s t test for which a p value <0.05 was defined as significant