Table 1 Primer sequences used in qPCR (5′->3′)
From: Inflammation-associated regulation of RGS in astrocytes and putative implication in neuropathic pain
Primer | Sequence | Expected size of the amplicon (bp) |
|---|---|---|
GAPDH forward | GTCTCCTGTGACTTCAACAG | 76 |
GAPDH reverse | AGTTGTCATTGAGAGCAATGC | |
RGS2 forward | TGCCCAAAATATCCAAGAGG | 205 |
RGS2 reverse | CGGGAGACAGAATGGAATGT | |
RGS3 forward | GTATCTTCGGGCTCATGGAA | 192 |
RGS3 reverse | TTACTTGTCCCCTCCGTCAC | |
RGS4 forward | TTCATCTCTGTGCAGGCAAC | 192 |
RGS4 reverse | GGAAGGATTGGTCAGGTCAA | |
RGS7 forward | TCGTCACATGAGAGCTGGAC | 158 |
RGS7 reverse | GACAGTGTCCCTTGGCAAAT |