Fig. 2
From: Role of microglial amylin receptors in mediating beta amyloid (Aβ)-induced inflammation
Amylin receptors on microglia in human Alzheimer’s disease brain and BV2 microglial cells. a, b Section from the inferior frontal gyrus of the AD patient (Braak Stage 6) after dual immunostaining both with the antibody to microglial marker Iba-1 (yellow) and calcitonin receptor (CTR) (dark blue). Arrows show microglial cells expressing both Iba-1 and CTR; thin arrows point to microglial cells expressing only Iba-1. c, d Single microglial cells are presented at a higher magnification, with thin arrows pointing to Iba-1 location (yellow) and arrows pointing to the CTR location (dark blue). e Coronal section from the inferior frontal gyrus of the AD patient after double immunohistochemical staining with antibody against CTR and receptor activity modifying protein 3 (RAMP3). Notice the microglia-like profile (mgl) expressing both CTR (blue) and RAMP3 (yellow). f BV2 microglial cells expressed CTR (red). g These BV2 cells were cultured in the presence of Aβ1–42 1 μM for 24 h. The Aβ was taken up into the cells and formed plaque-like structures stained with thioflavin S (green). Scale bars = 10 μm