Fig. 3
From: Role of microglial amylin receptors in mediating beta amyloid (Aβ)-induced inflammation
Amylin receptor mediates activation of inflammasomes in microglia. a Increased activation of the inflammasome, NLRP3 (green), following application of either Aβ1–42 or hAmylin human fetal microglial (HFMs) cultures. Aβ is identified with immunostaining with 6E10 antibody and shows as yellow on account of its co-localization with NLRP3. b BV2 cells (murine microglial cell line) treated either Aβ1–42 or hAmylin (2 μM) for 24 h show a similar increase in NLRP3 staining as in HFMs that is attenuated by 16 h pre-treatment with AC253 (10 μM). c Summary of amylin receptor mediated changes in NLRP3 observed and quantitated from Western blots, an example of which is shown in d. **p < 0.01. Scale bar = 20 μm