Fig. 4
From: Role of microglial amylin receptors in mediating beta amyloid (Aβ)-induced inflammation
Aβ induces upregulation of caspase-1 and pro-inflammatory cytokines in human fetal microglia (HFMs) via amylin receptor. a, b Aβ1–42 or hAmylin increase caspase-1 expression that is detected by a fluorometric assay (green). Pre-treatment of HFMs with amylin receptor antagonist, AC253 blocks caspase-1 expression. c RT-PCR detection of cytokines (TNFα, IL-10, IL-1β, and IL-23) in HFMs treated with either hAmylin, Aβ1–42, or AC253. Pre-treatment of HFMs with AC253 markedly attenuates upregulation of TNFα, IL-10, and IL-1β, but not IL-23. *p < 0.01. Scale bar = 20 μm