Fig. 4

Upregulation of KCa3.1, GFAP, and TRPV4 channels following OGD in cultured astrocytes. Western blot analysis of (a) KCa3.1, (b) GFAP, and (c) TRPV4 expression after OGD-treatment for 0, 1, 3, 4, 6, 12 h. Data represent the means ± SEM of KCa3.1, GFAP, and TRPV4 density normalized to β-actin values for n = 3 cultures. ^* p < 0.05, ^** p < 0.01, one-way ANOVA followed by the Dunnett’s multiple comparison test compared with control. (d) Cell viability was determined by the CCK-8 assay. The values represent the percentage of cells viability induced by 1, 3, 4, 6, or 12 h of OGD (n = 4). Data represent means ± SEM. ^* p < 0.05, ^*** p < 0.001, unpaired, two-tailed Student’s t test compared with control. Con control, OGD oxygen-glucose deprivation