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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Withania somnifera as a potential candidate to ameliorate high fat diet-induced anxiety and neuroinflammation

Fig. 3

ASH suppressed the synthesis and secretion of pro-inflammatory cytokines. a Histograms represent the serum levels of TNFα, IL-1β, and IL-6 among LFD, HFD, LFDE, and HFDE rats (n = 8 each group). b, c Representative Western blot analysis for TNFα, IL-1β, and IL-6 in the hippocampus and PC regions of rat brain among the four groups of animals. Histograms represent percent change in intensity taking value in LFD rats as 100%. d Histograms representing fold change in mRNA expression of TNFα and IL-1β in the hippocampus and PC regions of rat brain among the four groups of animals (n = 3–4 each group). Values are expressed as mean ± SEM. * p ≤ 0.05 LFD versus HFD, LFDE, and HFDE rats, # p ≤ 0.05 HFD versus HFDE rats, $ p ≤ 0.05 HFD versus LFDE rats, @ p ≤ 0.05 LFDE versus HFDE rats, Holm–Sidak method after one-way ANOVA

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