Fig. 4

Myelin loss and remyelination in wt, tg6, and tg21 mice during EAE. a Luxol fast blue myelin staining of lumbar and thoracic spinal cord from naïve wt mice and wt, tg21, and tg6 mice at late-stage EAE. Scale bar = 200 μm. b Representative Western blot analysis of CNS lysates from 2 naïve and 2 EAE diseased tg21, tg6 and wt mice for myelin basic protein (MBP). Reduced intensity of MBP bands at peak EAE indicates myelin loss, whereas the increase in intensity at late-stage EAE indicates remyelination