Fig. 5

Neuronal loss in the spinal cords of wt, tg6, and tg21 mice at late-stage EAE. a Immunohistochemical analysis of lumbar and thoracic spinal cord sections for neurofilament (NF), non-phosphorylated neurofilament H (smi32), and phosphorylated neurofilament H and M (smi35). Inserts in smi35 panels show DAPI co-stain revealing leukocyte infiltration into the spinal cord. Scale bar = 200 μm (b) Quantitative analysis of immunohistochemistry shown in a. NF: wt n = 4, tg21 n = 3; smi32: wt n = 3, tg21 n = 3; smi35: wt n = 4, tg21 n = 4. Four sections per animal from lumbar and thoracic spinal cord were analyzed. t test versus wt *p < 0.05, **p < 0.01