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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Elevated microRNA-129-5p level ameliorates neuroinflammation and blood-spinal cord barrier damage after ischemia-reperfusion by inhibiting HMGB1 and the TLR3-cytokine pathway

Fig. 5

Effect of the miR-129-5p mimic and mimic control on the expression of HMGB1 and TLR3-mediated cytokines at 48 h after IR. a Representative fluorescence images of the distribution of TLR3(red), HMGB1(green) and nuclei (blue) in the spinal cords at 48 h after IR. b Representative western blots of HMGB1 and TLR3 expression. GAPDH was used as a loading control. c Quantification of the relative protein expression of HMGB1 and TLR3 at 48 h after IR. d Quantification of the relative mRNA expression of HMGB1 and TLR3 at 48 h after IR. e Quantification of spinal IL-1β and TNF-α production at 48 h after IR. All data were obtained from three independent experiments and expressed as the mean ± SD. *P < 0.05 versus the Sham group. # P < 0.05 versus the IR group

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Journal of Neuroinflammation

ISSN: 1742-2094