Fig. 7

Effect of bone cancer inoculation and intrathecal T10 administration on HDAC1 in the spinal dorsal horn (SDH) on POD 14. a Ipsilateral SDH of the spinal sections showing the HDAC1 immunoreactive staining. The second row is the higher magnification images indicated in the white boxes in the first row. Scale bar 100 μm in the first row and 50 μm in the second row. b The percentage of HDAC1 staining surface in the laminae I–II and whole SDH on the ipsilateral side (n = 4). c Western blot analysis of the expression of HDAC1 in the SDH, relative to that of sham + vehicle group (n = 5). d Double-staining immunofluorescent images showing the distribution of HDAC1 (green) in neurons (NeuN, red), astrocytes (GFAP, red), and microglia (Iba-1, red) in different groups. Images in the white boxes are the amplification of an area in the corresponding image. Scale bar 100 μm outside of the white frame and 20 μm in the white frame. *p < 0.05 vs. sham + vehicle group; ^# p < 0.05 vs. BCP + vehicle group