Fig. 2From: DHCR24 exerts neuroprotection upon inflammation-induced neuronal deathDHCR24 overexpression increases neuronal viability without affecting TNFα, caspase-3, or NO levels in the neuron-BV2 cell co-cultures. a DHCR24 levels were significantly increased after lentivirus-mediated gene transfer. Molecular masses in kilodaltons are indicated on the left side of the blots. b Viability of mouse primary cortical neurons during LPS and IFN-γ treatment is significantly increased by DHCR24 overexpression. The viability of vehicle-treated neurons overexpressing DHCR24 does not differ from the control cells. c TNFα levels were significantly increased after LPS and IFN-γ treatment but unaffected by DHCR24 overexpression. d Pro-caspase-3-normalized activated caspase-3 levels were significantly upregulated after LPS and IFN-γ treatment, whereas DHCR24 overexpression had no effect on caspase-3 activation. Western blot of caspase-3 is shown in panel a. e NO levels did not significantly differ between DHCR24 overexpressing and control cells. NO levels in the vehicle-treated cells were undetectable. *p < 0.05, **p < 0.01, ***p < 0.001, mean ± SEM, n = 6 (a, b, d), and n = 15–17 (c, e)Back to article page