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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: DHCR24 exerts neuroprotection upon inflammation-induced neuronal death

Fig. 5

Overexpression of DHCR24 increases total and mushroom spine density in mouse hippocampal neurons. a Immunofluorescence staining of mouse hippocampal neurons (DIV20) with DHCR24 antibody. Neurons were transiently transfected with control + GFP plasmids (top row), or DHCR24 + GFP plasmids (lower row), where the white arrow points to the GFP and DHCR24-positive dendrite. Magnification of the GFP-positive cell soma and part of the dendrite inside the white box is shown next to the overlay picture. Magnification × 63, scale bars 20 μm. b Representative images of dendritic segments transiently transfected either with control or DHCR24 plasmid. Spine density quantification demonstrates that transient overexpression of DHCR24 significantly increases total spine density and mushroom spine density per micrometer in hippocampal neurons (DIV20). *p < 0.05, mean ± SEM, n = 40 (the total number of neurons analyzed from three independent experiments). Magnification 63×, zoom 3×. Scale bar 1 μm

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