Fig. 3

EZH2 inhibition in GBM cells weakens M2 phenotype of PBMC-derived macrophages. Human U87 GBM cells were incubated with siEZH2 and DZnep for 24 h and then co-cultured with PBMC-derived macrophages of M2 phenotype prior induced by cytokines M-CSF. Another 24 h later, macrophages were harvested for evaluation of CD206 (an M2 marker) by flow cytometry. Right half quadrant shows macrophages, whereas right upper shows M2 phenotype of macrophages. NC and PBS were set as controls and just medium were added to mock group. This experiment was repeated twice, and one representative result was illustrated