Fig. 5
From: EZH2 suppression in glioblastoma shifts microglia toward M1 phenotype in tumor microenvironment
EZH2 inhibition in GBM stimulates phagocytosis in microglia in an iNOS-dependent manner. a-d Murine GL261 cells were pre-treated with siEZH2 and DZNep for 24 h, and then, GL261 were removed and 1400 W, a specific iNOS inhibitor, was added to microglia for another 24 h with a working concentration of 500 μM. Next, fluorescent latex beads were added to microglia for 90 min. Amounts of latex beads phagocytized by microglia were detected by flow cytometry. NC was used as controls, and nothing was added to CON group. The experiments were repeated thrice, and one representative experiment was shown here