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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Peripheral immune tolerance alleviates the intracranial lipopolysaccharide injection-induced neuroinflammation and protects the dopaminergic neurons from neuroinflammation-related neurotoxicity

Fig. 2

Repeated low-dose LPS intraperitoneal injection-induced endotoxic tolerance of PBM. PBM from three groups with or without LPS pre-treatment in vivo were isolated and cultured. Then, TNF-α and IL-1β secreted by PBM and TLR4 expressed in PBM were quantified post to a single high-dose LPS (100 ng/ml) administration for 4 h in vitro. The productions of TNF-α and IL-1β were decreased and the expression of TLR4 in PBM was downregulated after LPS restimulation by repeated 0.3 mg/kg LPS intraperitoneal injection in LPS (i.p.) group. a, b The levels of TNF-α (a) and IL-1β (b) in the supernatant were detected by ELISA. c, d The expression of TLR4 in PBM was quantified by measuring band intensities using ImageJ software. The values were normalized to GAPDH. **P < 0.01, ***P < 0.001 vs. control group. #P < 0.05, ###P < 0.001 vs. PBS (i.p.) group. The data are presented as the mean ± SEM (n = 6)

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Journal of Neuroinflammation

ISSN: 1742-2094