Fig. 10

Minocycline improves glia cells morphology and cells count in hippocampus following temozolomide administration during SD. Changes in the a soma density. b Soma area. c Sum inters. d Mean inters. e Ramification index. f Astrocyte length of astrocyte in DG, CA1, and CA3 regions of the hippocampus. Changes in the g soma density. h Soma area. i Sum inters. j mean inters. k Ramification index. l Microglial length of microglia in DG, CA1, and CA3 regions of the hippocampus. Changes in the microglial cell count at different stages (resting, intermediate, and activated) in m DG region. n CA1 region. o CA3 region of the hippocampus. Total microglia cell count in p DG region. q CA1 region. r CA3 region of the hippocampus. ^α p < 0.05 when sleep deprived treated with TMZ compared to control treated with TMZ. ^β p < 0.05 when sleep deprived treated with TMZ and minocycline compared to sleep deprived treated with TMZ. One-way ANOVA with Bonferroni post hoc test was used for the analysis of soma density, soma area, astrocyte length, and microglia size while Kruskal-Wallis with Dunn’s post hoc test was applied for sum inters, mean inters, ramification index parameters, and cell count analysis