Fig. 6

Minocycline improves SD-induced alteration in the glia cells morphology and cell count in rat hippocampus. Representable image (intersection and segmented mask) of a normal and activated astrocyte. b Resting, intermediate, and activated stage microglial cell. Changes in the c soma density. d Soma area. e Sum inters. f Mean inters. g Ramification index. h Astrocyte length of astrocyte in DG, CA1, and CA3 regions of the hippocampus. Changes in the i soma density. j Soma area. k Sum inters. l Mean inters. m Ramification index. n Microglial length of microglia in DG, CA1, and CA3 regions of the hippocampus. Changes in the microglial cell count at different stages (resting, intermediate, and activated) in o DG region. p CA1 region. q CA3 region of the hippocampus. Total microglia cell count in r DG region. s CA1 region. t CA3 region of the hippocampus.*p < 0.05 when compared to control treated with vehicle. ^ϕ p < 0.05 when compared to sleep deprived treated with vehicle. One-way ANOVA with Bonferroni post hoc test was used for the analysis of soma density, soma area, astrocyte length, and microglia size while Kruskal-Wallis with Dunn’s post hoc test was applied for sum inters, mean inters, ramification index parameters, and cell count analysis