Isolated primary microglia from GrnKO mice display a hyper-inflammatory phenotype that is not present in Lyz-cKO mice.
a Measurement of Grn by ELISA in the conditioned media from primary microglia cultures. b Measurement of the inflammatory cytokine IL-6 in the conditioned media of primary microglia cultures after 24 h of stimulation with controlled standard endotoxin (CSE). N = 4–9 wells per genotype derived from two independent experiments. Genotypes are defined as follows: WT: Grn+/+, Lyz+/+; WT-Cre: Grn+/+, Lyzcre/cre; Het: Grn+/−; Lyz-cKO: Grnflox/flox, Lyzcre/cre; KO-Lyz-cKO: Grnflox/−, Lyzcre/cre; GrnKO: Grn−/−. Data represent mean ± SEM. p values were calculated using Tukey’s multiple comparison test after one-way ANOVA