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Table 1 Primer sequences for genotyping and quantitative RT-PCR

From: Selective depletion of microglial progranulin in mice is not sufficient to cause neuronal ceroid lipofuscinosis or neuroinflammation

Genotyping

Gene

Forward primer sequence

Reverse primer sequence

Grn

Common:

5’-CGGAACACAGTGTCCAGATG-3’

Intron 2:

5’-ATCAACCAAAGGGTCTGTGC-3’

Exon 5:

5’-GTGGCAGAGTCAGGACATTCAAACT-3’

Lys2

WT: 5’-TTACAGTCGGCCAGGCTGAC-3’

Cre: 5’-CCCAGAAATGCCAGATTACG-3’

Common:

5’- CTTGGGCTGCCAGAATTTCTC-3’

Quantitative RT-PCR

Gene

Forward primer sequence

Reverse primer sequence

CD11b

5’-ATCCCCCTGCAAGACAGTGA-3’

5’-AGCAGTCAGGCAGGGACATG-3’

CD68

5’-GTGCTCATCGCCTTCTGCATCA-3’

5’-GGCGCTCCTTGGTGGCTTAC-3’

Gfap

5’-GAGTGGTATCGGTCTAAGTTTGCA-3’

5’-CGATAGTCGTTAGCTTCGTGCTT-3’

Grn

5’-CTGTAGTGCAGATGGGAAATCCTGCT-3’

5’-GTGGCAGAGTCAGGACATTCAAACT-3’

Iba1

5’-GTCCTTGAAGCGAATGCTGG-3’

5’-CATTCTCAAGATGGCAGATC-3’

Lamp1

5’-ACATCAGCCCAAATGACACA-3’

5’-GGCTAGAGCTGGCATTCATC-3’

Lamp2

5’-AGCACAGTATTTCCTGGTGCT-3’

5’-CGACAGGAGTCAGGTTGTAAGTTAA-3’

RplI13a

5’-GGAGGAGAAACGGAAGGAAAAG-3’

5’-CCGTAACCTCAAGATCTCGTTCTT-3’

Usf1

5’-CCTGTGGCGTGGCAGTCT-3’

5’-TGCACGCCCACACTGTTT-3’

Zfp91

5’-TCCTGTGGGCGACTCTTCAG-3’

5’-TAATCCCTCTGGTCTGTATGATGCT-3’