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Table 1 Primer sequences for genotyping and quantitative RT-PCR

From: Selective depletion of microglial progranulin in mice is not sufficient to cause neuronal ceroid lipofuscinosis or neuroinflammation

Genotyping
Gene Forward primer sequence Reverse primer sequence
Grn Common:
5’-CGGAACACAGTGTCCAGATG-3’
Intron 2:
5’-ATCAACCAAAGGGTCTGTGC-3’
Exon 5:
5’-GTGGCAGAGTCAGGACATTCAAACT-3’
Lys2 WT: 5’-TTACAGTCGGCCAGGCTGAC-3’
Cre: 5’-CCCAGAAATGCCAGATTACG-3’
Common:
5’- CTTGGGCTGCCAGAATTTCTC-3’
Quantitative RT-PCR
Gene Forward primer sequence Reverse primer sequence
CD11b 5’-ATCCCCCTGCAAGACAGTGA-3’ 5’-AGCAGTCAGGCAGGGACATG-3’
CD68 5’-GTGCTCATCGCCTTCTGCATCA-3’ 5’-GGCGCTCCTTGGTGGCTTAC-3’
Gfap 5’-GAGTGGTATCGGTCTAAGTTTGCA-3’ 5’-CGATAGTCGTTAGCTTCGTGCTT-3’
Grn 5’-CTGTAGTGCAGATGGGAAATCCTGCT-3’ 5’-GTGGCAGAGTCAGGACATTCAAACT-3’
Iba1 5’-GTCCTTGAAGCGAATGCTGG-3’ 5’-CATTCTCAAGATGGCAGATC-3’
Lamp1 5’-ACATCAGCCCAAATGACACA-3’ 5’-GGCTAGAGCTGGCATTCATC-3’
Lamp2 5’-AGCACAGTATTTCCTGGTGCT-3’ 5’-CGACAGGAGTCAGGTTGTAAGTTAA-3’
RplI13a 5’-GGAGGAGAAACGGAAGGAAAAG-3’ 5’-CCGTAACCTCAAGATCTCGTTCTT-3’
Usf1 5’-CCTGTGGCGTGGCAGTCT-3’ 5’-TGCACGCCCACACTGTTT-3’
Zfp91 5’-TCCTGTGGGCGACTCTTCAG-3’ 5’-TAATCCCTCTGGTCTGTATGATGCT-3’