Genotyping | ||
Gene | Forward primer sequence | Reverse primer sequence |
Grn | Common: 5’-CGGAACACAGTGTCCAGATG-3’ | Intron 2: 5’-ATCAACCAAAGGGTCTGTGC-3’ Exon 5: 5’-GTGGCAGAGTCAGGACATTCAAACT-3’ |
Lys2 | WT: 5’-TTACAGTCGGCCAGGCTGAC-3’ Cre: 5’-CCCAGAAATGCCAGATTACG-3’ | Common: 5’- CTTGGGCTGCCAGAATTTCTC-3’ |
Quantitative RT-PCR | ||
Gene | Forward primer sequence | Reverse primer sequence |
CD11b | 5’-ATCCCCCTGCAAGACAGTGA-3’ | 5’-AGCAGTCAGGCAGGGACATG-3’ |
CD68 | 5’-GTGCTCATCGCCTTCTGCATCA-3’ | 5’-GGCGCTCCTTGGTGGCTTAC-3’ |
Gfap | 5’-GAGTGGTATCGGTCTAAGTTTGCA-3’ | 5’-CGATAGTCGTTAGCTTCGTGCTT-3’ |
Grn | 5’-CTGTAGTGCAGATGGGAAATCCTGCT-3’ | 5’-GTGGCAGAGTCAGGACATTCAAACT-3’ |
Iba1 | 5’-GTCCTTGAAGCGAATGCTGG-3’ | 5’-CATTCTCAAGATGGCAGATC-3’ |
Lamp1 | 5’-ACATCAGCCCAAATGACACA-3’ | 5’-GGCTAGAGCTGGCATTCATC-3’ |
Lamp2 | 5’-AGCACAGTATTTCCTGGTGCT-3’ | 5’-CGACAGGAGTCAGGTTGTAAGTTAA-3’ |
RplI13a | 5’-GGAGGAGAAACGGAAGGAAAAG-3’ | 5’-CCGTAACCTCAAGATCTCGTTCTT-3’ |
Usf1 | 5’-CCTGTGGCGTGGCAGTCT-3’ | 5’-TGCACGCCCACACTGTTT-3’ |
Zfp91 | 5’-TCCTGTGGGCGACTCTTCAG-3’ | 5’-TAATCCCTCTGGTCTGTATGATGCT-3’ |