Fig. 2
From: Dendrimer-mediated delivery of N-acetyl cysteine to microglia in a mouse model of Rett syndrome
Increased nitric oxide (NO) release from Mecp2-null primary mixed glial cells challenged with LPS. Cortical tissue samples from the brains of 1-week-old Mecp2-null and WT mice were taken, and cells were dissociated and plated to grow. Once confluent, cells were plated, activated with LPS, and treated with D-NAC and NAC (left panel). Both WT and Mecp2-null glial cells showed equivalent NO release in response to LPS (black bars) that was diminished significantly by D-NAC (blue bars) administration (right panel). Only the high dose of free NAC was effective in decreasing the response to LPS (red bars)