Fig. 2
Deletion of sEH reduced microglia/macrophage activation and expression of proinflammatory mediators and EET degradation after ICH. a The experimental design scheme used to study the effect of sEH deletion. ELISA enzyme-linked immunosorbent assay, EB Evans blue. b Iba1 staining and quantitative data at 1 and 4 days post-ICH. The inset is a representative activated microglia/macrophage at higher magnification. Low magnification of a cresyl violet-stained brain section of a core hemorrhagic region at 0.24 mm from the bregma (bottom right). The white box indicates the location of representative images. The number of Iba1-positive cells is expressed as the mean number per field of view (0.8 mm2). The scale bar is 100 μm. Bar graphs of c IL-1β, d IL-6, e MIP-2, f MCP-1 protein levels, and g EET protein level, EET/14,15-DHET ratio, and 14,15-DHET protein level, as assessed by ELISA at 1 and 4 days post-ICH. Values are mean ± S.E.M.; #, †P < 0.05, ##, ††P < 0.01, and ###, †††P < 0.001 vs. sham group; *, § P < 0.05, **P < 0.01, and ***, §§§ P < 0.001 vs. WT group (n = 6 mice/group for Iba1 staining, Student’s t test; n = 4–6 mice/group for ELISA, repeated measures two-way ANOVA)