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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Genetic deletion or pharmacological inhibition of soluble epoxide hydrolase reduces brain damage and attenuates neuroinflammation after intracerebral hemorrhage

Fig. 7

Pharmacological inhibition of sEH reduced P38 MAPK and P65 NF-κB activation in cultured microglia and attenuated microglia-mediated neurotoxicity in vitro. Representative immunoblots and quantitative data of a P65, b P38, c JNK, and d ERK phosphorylation following co-treatment of 10 μM AUDA with thrombin or hemin in BV2 microglia. e Experimental scheme of neuronal cell death in N2A cells in response to thrombin- or hemin-treated BV2-conditioned media with or without 10 μM AUDA pretreatment. BV2 microglia were incubated with thrombin or hemin in the absence or presence of 10 μM AUDA for 24 h. N2A cells were treated for 48 h with cell-free supernatant fractions derived from BV2 microglia and cell viability was evaluated. f Neuronal cell viability as assessed by the MTT assay after exposure to thrombin- or hemin-treated conditioned microglial media. Values are mean ± S.E.M.; # P < 0.05, ## P < 0.01, and ### P < 0.001 vs. control group; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. thrombin- or hemin-stimulation group (n = 4–5 experiments/group for Western blot, n = 4 experiments/group for cell viability, one-way ANOVA)

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