Fig. 9

Chronic DMXBA treatment reverses chronic restraint stress (CRS)-induced reductions in T regulatory (Treg) cells in mouse periphery and hippocampus. a Representative flow cytometry profiles and statistics of Foxp3⁺-expressing cells among CD4⁺CD25⁺ splenocytes on days 7, 14, and 21 of CRS. b Representative flow cytometry profiles and statistics of Treg cells among splenocytes from mice treated with vehicle or DMXBA. c Representative immunoblots and relative levels of Foxp3 protein in hippocampus. Results are shown as fold change relative to GAPDH protein level. Con, control group. All data are expressed as mean ± SEM. n = 8; *P < 0.05 vs. control group; ^# P < 0.05, ^## P < 0.01 vs. CRS 21d + Veh group; ⁺⁺ P < 0.01 vs. CRS 21d + DMXBA group