Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Asiatic acid attenuates methamphetamine-induced neuroinflammation and neurotoxicity through blocking of NF-kB/STAT3/ERK and mitochondria-mediated apoptosis pathway

Fig. 2

AA suppresses the translocation of NF-κB and STAT3 in METH-stimulated SH-SY5Y cells. a Immunoblot analysis shows the effects of 20 μM AA on the inhibition of translocation of NF-κB/STAT3 and phosphorylation of JAK2 (an upstream activator of STAT3) in 1 mM METH-stimulated SH-SY5Y cells. β-actin and lamin B were used to confirm equal sample loading. DNA- and Ab-binding activity of NF-κB (b) and STAT3 (c) in nuclear extracts was measured by EMSA and supershift assay. d Immunofluorescence double staining for p-NF-κB p65 (green) and p-STAT3 (red) localization. Cells were counterstained with Hoechst 33342 (blue). Magnifications ×200. Immunoblotting, EMSA, and supershift assay were quantified by densitometric analysis. The data are representative of three independent experiments and quantified as mean values ± SEM (n = 3 to 4). Tukey’s multiple comparison test, *p < 0.05 compared to normal control, p < 0.05 compared to METH treatment

Back to article page