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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Asiatic acid attenuates methamphetamine-induced neuroinflammation and neurotoxicity through blocking of NF-kB/STAT3/ERK and mitochondria-mediated apoptosis pathway

Fig. 6

AA protects METH-induced apoptosis in SH-SY5Y cells. a Annexin V/PI staining by flow cytometry. AA inhibits cleavages of caspase-3 and PARP (b) and regulates apoptotic proteins (c). VDAC was used as mitochondrial loading control, and β-actin was used to confirm equal sample loading. Immunoblotting was quantified by densitometric analysis. SH-SY5Y cells were evaluated by morphological criteria after JC-1 mitochondria staining (red and green) by using fluorescent microscope (upper) and flow cytometry (lower). Magnifications ×200. The data are representative of three independent experiments and quantified as mean values ± SEM (n = 3 to 9). Tukey’s multiple comparison test, *p < 0.05 compared to normal control, p < 0.05 compared to METH treatment

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