Fig. 1

SSO prevented inflammation-induced cell viability alteration and NO release. SSO alone did not alter the viability of BV2 cells. Exposure of BV2 cells to 100 ng/ml LPS and 5 ng/ml IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with SSO prevented it as revealed by resazurin assay (a). The values are normalized to control. In addition, LPS/IFNγ treatment induced a massive NO production in BV2 cells which was blocked by co-treatment with SSO at both concentrations as measured by Griess reaction (b). The results were analyzed with one-way ANOVA followed by Tukey’s post hoc test. Data are expressed as mean ± SEM. n = 3 per group from three independent experiments. *p < 0.01, **p < 0.01 and ***p < 0.001