Fig. 5
From: Selective proliferative response of microglia to alternative polarization signals
In vivo effects of M2 polarization signals on microglia gene expression. Microglia were isolated by immunosorting 24 h after a i.c.v. injections of vehicle (veh; open boxes), 5 μg IL-4 (closed boxes) or 1 μg CSF-1 (gray boxes) or b s.c. 17β-estradiol (E2) treatment (5 μg/kg). The expression of genes related with active replication and cell cycle (Ki67; Cdk1, Ccnb2) and M2 alternative polarization (Arg1, Fizz1, Ym1) was analyzed by real-time PCR. Data sets for each gene were calculated using the 2-ddCt method with respect to the mean value of the vehicle group. Bars represent mean values ± SEM (n = 4). Student’s unpaired t test, *p < 0.05; **p < 0.01; ***p < 0.001 versus veh