Fig. 7
Reversibility of the AM404 mediated inhibition of COX-1 (a) and COX-2 (b) activity in primary rat microglia cells. a The cells were treated with different concentrations of AM 404 (0.1, 1, 5, or 10 μM) for 60 min. For COX-2 (b), cells were stimulated for 24 h with LPS (10 ng/mL) and then treated with different concentrations of AM 404 for 60 min. After this incubation time, a and b were washed three times with DPBS 37 °C and incubated for 3 h with serum-free medium. Fifteen micromolar of arachidonic acid was added, and PGE2 in the supernatants was measured as described in “Methods.” Data are expressed as mean ± SEM of at least four new cultures/group). *p < 0.05 and **p < 0.01 with respect to control (one-way ANOVA followed by the Newman-Keuls post-test)