Fig. 4

Lymphocyte infiltration persists in the ischemic brain at day 14 after onset in mice. a Lymphocytes were isolated from spleens of C57BL/6 (B6) mice and co-cultured with MIRB. 2 × 10⁷ MIRB-labeled cells were then adoptively transferred into Rag2^−/−γc^−/− mice followed by sham or stroke procedures. MRI images show hypointensive dots that indicate MIRB-labeled lymphocytes in the ischemic brains of Rag2^−/−γc^−/− mice. b Summarized results show signal of MIRB-labeled lymphocytes in mice receiving either sham or MCAO operations. n = 8 mice per group. c Dot plots of flow cytometry assay show CD4⁺ T, CD8⁺ T, NK, and B cells in single cell suspension from brains of photothrombosis- or MCAO-operated mice at 14 days after stroke. d Bar graphs summarize the cumulative data for quantifying CD4⁺ T, CD8⁺ T, NK, and B cell population from brains of photothrombosis- or MCAO-operated mice at 14 days after stroke. n = 8 mice per group. e At 14 day after surgery, representative immunostaining images show infiltrated CD4⁺ T, CD8⁺ T, and NK cells around ischemic lesion area in photothrombosis and MCAO mice. Red: CD4, CD8 or NKp46; blue: DAPI. Scale bars: 40 μm, 20 μm (inset). f Quantification analysis shows the infiltrated CD4⁺ T, CD8⁺ T, and NK cell counts from ipsilateral brain in photothrombosis and MCAO mice 14 days after ischemia. n = 5 mice per group. Error bars represent s.e.m.; *p < 0.05; **p < 0.01, sham vs. stroke group (photothrombosis/MCAO) by one-way ANOVA