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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Alpha-synuclein oligomer-selective antibodies reduce intracellular accumulation and mitochondrial impairment in alpha-synuclein exposed astrocytes

Fig. 1

Oligomer-selective antibodies reduce α-syn oligomer accumulation in astrocytes. a α-syn oligomers incubated with mAb47 (0.05 μM, 1:1 ratio) 1 h at 37 °C were analyzed with mab38F/biot-mab38F sandwich ELISA. The addition of mAb47 resulted in a strong reduction of the α-syn oligomer signal, implying steric hindrance by mAb47 due to high Ab:α-syn oligomer complex formation. b Cells were exposed to fluorescent α-syn oligomers or to oligomers pre-incubated with the α-syn oligomer-selective antibodies: mAb47, mAb49G, or mAb38E2, for 24 h. The α-syn accumulation was strongly reduced when oligomers had been pre-incubated with antibodies. The mAb47 had the most pronounced reducing effect on α-syn oligomer accumulation. Measurements of the intracellular Cy3-labeled α-syn, using the Zen-software, confirmed that the c area/cell and d intensity/cell were significantly decreased in the antibody-treated cultures. Scale bars = 10 μm. Data are presented as mean ± SD from three independent experiments and levels of significance were set to *P < 0.05, **P < 0.01, and ***P < 0.001

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