Fig. 8

The presence of mAb47 slows down astrocytic accumulation of α-syn oligomers. Co-cultures were exposed to either Cy3-α-syn oligomers or to mAb47-treated oligomers and monitored with time-lapse imaging for 24 h. Areas highlighted with white rectangles to the left (20x) are displayed at higher magnification to the right (40x). When cells were exposed to oligomers alone, the astrocytic accumulation of fluorescent α-syn (red) was rapid, with deposits detectable already after 15 min that were increasing throughout the experiment (a). Exposure to mAb47-treated oligomers led to a much lower accumulation in astrocytes. At 24 h, a weak Cy3-α-syn signal was visible in the astrocytes (b). Moreover, no free-floating fluorescent aggregates were observed in the cell media, suggesting an overall increased clearance of α-syn oligomers (scale bars = 10 μm)