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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Contrasting effect of the latency-reversing agents bryostatin-1 and JQ1 on astrocyte-mediated neuroinflammation and brain neutrophil invasion

Fig. 1

HIV-1 infection promotes secretion of CCL2, IL-6, IL-8, and GM-CSF by astrocytes. Primary human astrocytes were either left uninfected or infected with VSV-G-pseudotyped eGFP-encoding HIV-1 particles for 7 days. a Infection was assessed by monitoring the percentage of eGFP-expressing astrocytes by flow cytometry. Data from one representative donor is shown. Next, cell-free supernatants were collected to measure b CCL2, c IL-6, d IL-8, and e GM-CSF production by commercial ELISA tests. Results are presented in raw data (i.e., cytokine levels expressed in pg/mL or ng/mL). Data from six to nine experiments with independent donors are shown. Each colored symbol represents a different donor (the same color is preserved for the same donor throughout our study), and the horizontal line represents the grand mean. Asterisks denote statistically significant data as defined by the Student t test (*P < 0.05 and **P < 0.01)

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