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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Contrasting effect of the latency-reversing agents bryostatin-1 and JQ1 on astrocyte-mediated neuroinflammation and brain neutrophil invasion

Fig. 5

ACM from bryostatin-1- and JQ1-treated astrocytes affects neutrophil transmigration through an in vitro BBB model. a Schematic representation of the in vitro BBB model and neutrophil transmigration assay. Neutrophil transmigration was assessed using BBB containing b, c astrocytes and the human BMVEC line hCMEC/D3 or d, e hCMEC/D3 cells alone, in response to either b, d ACM from LRA-treated astrocytes or c, e LRA added in ACM from untreated astrocytes. f Neutrophils were pretreated with of reparixin (1 μM) for 45 min before performing the transmigration assay with ACM from LRA-treated astrocytes (circles: without reparixin: squares: with reparixin). The results are expressed in percentage of transmigrated neutrophils. Each colored dot represents a different sample. Asterisks denote statistically significant data as defined by the Student t test (*P < 0.05 and **P < 0.01)

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