Fig. 1

Effects of pre supplementation of vitamin B6 on APE1, AIF, and Casp-3 mRNA expression levels. Gene expression was determined in the CX (a, b, c) and HC (d, e, f) by real-time PCR and the fold change was calculated using the ΔCq method. Values were transformed to percentage using the control group at 20 h post infection as 100%. Each column data expresses the mean percentage (± SEM) relative to control 20 h. Comparisons between columns which show p < 0.05 were significant in Dunn’s post test. Groups: uninfected control animals (CTRL) 20 h (n = 5), uninfected control animals (CTRL) 24 h (n = 5), infected animals (PM) without vitB6 treatment 20 h p.i. (n = 5), infected animals (PM) without vitB6 treatment 24 h p.i. (n = 5), infected animals plus vitB6 treatment (PM/pre sup vitB6) 20 h p.i. (n = 8), infected animals plus vitB6 treatment (PM/pre sup vitB6) 24 h p.i. (n = 10)