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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: IL-33/ST2 signaling contributes to radicular pain by modulating MAPK and NF-κB activation and inflammatory mediator expression in the spinal cord in rat models of noncompressive lumber disk herniation

Fig. 1

Silencing of the IL-33 gene by lentivirus-mediated delivery of shRNA in astroglial cells in vitro. a Fluorescence microscopy showed GFP fluorescence expression in nearly all the cells, which indicated a high and stable transduction of the lentiviral vector system. Bars = 100 μm. b Fluorescence microscopy images of astroglial cells 72 h after transduction with LV-shIL-33. Bars = 100 μm. c Representative real-time PCR of IL-33 mRNA expression in astrocytes in vitro. The fold change of the IL-33 levels in the control group was set as 1.0 for the quantifications. d Representative Western blots of the IL-33 protein expression in astroglial cells in vitro. β-tubulin was used as a loading control. The results are expressed as the ratio of IL-33 to β-tubulin, and the control was set at 1.0. The values are the mean ± SEM (**p < 0.01 versus the LV-NC group or the vehicle group, n = 6 in each group)

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