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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: IL-33/ST2 signaling contributes to radicular pain by modulating MAPK and NF-κB activation and inflammatory mediator expression in the spinal cord in rat models of noncompressive lumber disk herniation

Fig. 4

Spinally delivered lentiviral vector LV-shIL-33 inhibits the expression of IL-33 in the dorsal spinal cord. a Representative real-time PCR of IL-33 mRNA expression in the rat ipsilateral spinal dorsal horns tissue. The fold change of IL-33 levels in the sham group was set as 1.0 for the quantifications (n = 6 in each group). b Representative Western blots of the IL-33 protein expression in the rat ipsilateral spinal dorsal horn tissue. β-tubulin was used as a loading control. The results are expressed as the ratio of IL-33 to β-tubulin, and the control was set at 1.0 (n = 6 in each group). c Quantification of immunofluorescence signals for IL-33 in the rat ipsilateral spinal dorsal horns (n = 4–5 in each group). d Representative immunofluorescence detection of IL-33 in the rat ipsilateral spinal dorsal horns. Panels fj are higher-power magnifications of the boxed areas in the panels ae. Bars = 50 μm (n = 6 in each group). The values are the mean ± SEM (##p < 0.01 versus sham; **p < 0.01 versus the LV-NC group or the vehicle group)

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