Fig. 5

Effect of intra-TG etanercept administration on mechanical allodynia following IANX. a The timing of the cannula implantation, etanercept, or vehicle administration into the TG, IANX, or sham operation (upper) and changes in MHWT of the ipsilateral whisker pad skin by daily etanercept administration into TG following IANX (lower). The MHWT was normalized to the pre-IANX MHWT (100%). Arrows indicate the timing of intra-TG etanercept or vehicle administration. Error bars indicate SEM. **p < 0.01 vs. IANX + vehicle (n = 6 in each; two-way ANOVA with repeated measures followed by Bonferroni’s multiple-comparison test). b TNFR1 immunoreactivity in ipsilateral TG on day 3 following IANX or in sham-operated rats. Arrows indicate FG-labeled TNFR1-IR neurons. c Changes in the mean percentage of FG-labeled TNFR1-IR neurons on day 3 following IANX. Error bars indicate SEM. *p < 0.05 vs. sham (n = 5 in each, Student’s t test)